Analysis of a Growth-Promoting Factor for Babesia caballi Cultivation

H. Ikadai; M. D. Martin; H. Nagasawa; K. Fujisaki; N. Suzuki; T. Mikami; N. Kudo; T. Oyamada; I. Igarashi

doi:10.1645/0022-3395(2001)087[1484:AOAGPF]2.0.CO;2

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H. Ikadai,¹ M. D. Martin,² H. Nagasawa,³ K. Fujisaki,³ N. Suzuki,³ T. Mikami,³ N. Kudo,¹ T. Oyamada,¹ I. Igarashi^3,4

¹Department of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan.
²Ministry of Agriculture, Livestock, and Rural Development, Ukunda-Veterinary Investigation Laboratory, P.O. Box 70, Ukunda, Kenya.
³National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
⁴To whom correspondence should be addressed.

Abstract

Serum-free media were examined to culture Babesia caballi. Daigo's T (DT) basal medium supplemented with Daigo's GF21 (GF21) or GIT medium, which already contains GF21, supported the parasite propagation at 37 C in a humidified atmosphere under 5% CO₂ in air. Growth of B. caballi was dependent of the suitable concentration (10–20%) of GF21. Therefore, GF21 was suggested as the growth-promoting factor for B. caballi. However, GIT medium did not support the growth of parasites from cryopreserved stabilates, and serum supplementation was essential for the retrieval of parasites.

Citation Download Citation

H. Ikadai, M. D. Martin, H. Nagasawa, K. Fujisaki, N. Suzuki, T. Mikami, N. Kudo, T. Oyamada, and I. Igarashi "Analysis of a Growth-Promoting Factor for Babesia caballi Cultivation," Journal of Parasitology 87(6), 1484-1486, (1 December 2001). https://doi.org/10.1645/0022-3395(2001)087[1484:AOAGPF]2.0.CO;2

Published: 1 December 2001

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